GALC Antibody

• Catalog Number: orb1264795
• Category: Antibodies
• Description: GALC Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, WB
• Predicted Reactivity: Mouse
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This GALC antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 26-56 amino acids from human GALC.
• Concentration: batch dependent
• Dilution range: For FACS starting dilution is: 1:25For WB starting dilution is: 1:2000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 77 kDa
• Target: GALC
• UniProt ID: P54803
• NCBI: P54803
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Galactocerebrosidase, GALCERase, 3.2.1.46, Galacto
• Note: For research use only
• Application notes: For FACS starting dilution is: 1:25For WB starting dilution is: 1:2000
• Expiration Date: 12 months from date of receipt.

Overlay histogram showing Hela cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37oC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed (OH191631) at 1/200 dilution for 40 min at 37oC. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.

Western Blot at 1:2000 dilution Lane 1: human brain lysate Lane 2: Hela whole cell lysate Lane 3: NCI-H1299 whole cell lysate Lysates/proteins at 20 ug per lane.