Galanin antibody

• Catalog Number: orb20373
• Category: Antibodies
• Description: Goat polyclonal antibody to Galanin
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Bovine, Human, Mouse, Rat
• Dilution range: ELISA: 1:32000, WB: 1-3 μg/ml, IHC-P: 0.2-0.5 μg/ml
• Conjugation: Unconjugated
• MW: 13.3
• Target: GAL
• Entrez: 51083
• Protein Sequence: HRSFSDKNGLTSK
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti-GAL 1 antibody, anti- GAL antibody, anti- GAL
• Note: For research use only
• Application notes: IHC: Frozen section of Human Hypothalamus shows staining of galaninergic axons in the human periventricular nucleus. Recommended concentration, 0.2-0.5μg/ml.WB: Approx 17kDa band observed in Human Brain (Cerebellum) and Mouse Brain lysates (calculated MW of 13.3kDa according to NP_057057.2). Recommended concentration: 1-3 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb20373 Negative Control showing staining of paraffin embedded Human Small Intestine, with no primary antibody.

orb20373 (8µg/ml) staining of paraffin embedded Human Small Intestine. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

orb20373 (1µg/ml) staining of Mouse Brain (A) Small Intestine (B) and Stomach (C) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Western blot analysis of human cerebellum lysate (35µg protein in RIPA buffer) using GAL antibody

Immunohistochemical analysis of formalin-fixed paraffin embedded human hypothalamus tissue using GAL antibody

Western blot analysis of Mouse and Rat Brain lysate using Galanin antibody