GAGED2 Antibody

• Catalog Number: orb1246773
• Category: Antibodies
• Description: GAGED2 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: CGFGFRRQGEDNT
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Not yet tested - our routinely used western blotting protocol does not allow detection of proteins as small as the predicted size of approx. 8.0kDa according to NP_597673. Therefore we cannot recommend an optimal concentration and the product is investigative grade. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human testis shows polar cytoplasm staining in primary spermatocytes. Recommended concentration, 3-6ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: GAGED2
• UniProt ID: Q9HD64
• NCBI: NP_597673.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: GAGED2, XAGE1, XAGE-1, G antigen, family D, 2, XAG
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Not yet tested - our routinely used western blotting protocol does not allow detection of proteins as small as the predicted size of approx. 8.0kDa according to NP_597673. Therefore we cannot recommend an optimal concentration and the product is investigative grade. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human testis shows polar cytoplasm staining in primary spermatocytes. Recommended concentration, 3-6ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1246773 (3.8 ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.