GAD1 Antibody

• Catalog Number: orb1246607
• Category: Antibodies
• Description: GAD1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-PDSPQRREKLHK
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 65kDa band observed in Human Brain (Cerebral Cortex) lysates (calculated MW of 66.9kDa according to NP_000808.2). Recommended concentration: 0.5-1.5ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse and Rat Brain for this particular batch.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: GAD1
• UniProt ID: Q99259
• NCBI: NP_000808.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: GAD1, glutamate decarboxylase 1 (brain, 67kDa), FL
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 65kDa band observed in Human Brain (Cerebral Cortex) lysates (calculated MW of 66.9kDa according to NP_000808.2). Recommended concentration: 0.5-1.5ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse and Rat Brain for this particular batch.
• Expiration Date: 12 months from date of receipt.

orb1246607 (0.5 ug/ml) staining of Human Brain (Cerebral Cortex) lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1246607 (4 ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.