GABRA4 antibody

• Catalog Number: orb20137
• Category: Antibodies
• Description: Goat polyclonal antibody to GABRA4
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Bovine, Human
• Dilution range: ELISA: 1:16000, WB: 0.1-0.3 μg/ml, IHC-P: 2-4µg/ml
• Conjugation: Unconjugated
• MW: 61.6
• Target: GABRA4
• Entrez: 2557
• Protein Sequence: EKAKRKTSKPPQE
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti GABRA4 antibody, anti gamma-aminobutyric acid
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.IHC: In paraffin embedded Human Cortex shows patchy staining near or at the neuronal cell membrane.. Recommended concentration, 2-4ug/ml.WB: Approx. 70kDa band observed in Human Brain (Cerebellum and Frontal Cortex) lysates (calculated MW of 61.6kDa according to NP_000800.2). Recommended concentration: 0.1-0.3 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb20137 (0.1ug/ml) staining of Human Frontal Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb20137 (2.5ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.