G6PD Antibody (clone AT2F6)

• Catalog Number: orb1538093
• Category: Antibodies
• Description: G6PD Antibody (clone AT2F6)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: AT2F6
• Tested applications: ELISA, IHC, IHC-P, WB
• Reactivity: Human
• Isotype: IgG2b,k
• Immunogen: Recombinant human G6PD (35-506aa) purified from E. coli
• Concentration: 1 mg/ml
• Dilution range: ELISA, IHC, IHC-P (5 µg/ml), WB (1:1000 - 1:2000)
• Conjugation: Unconjugated
• Target: G6PD
• Storage: Aliquot and store at -20°C. Avoid freeze-thaw cycles.
• Buffer/Preservatives: PBS, pH 7.4, 0.1% Sodium Azide. PBS, pH 7.4, 0.1% Sodium Azide
• Alternative names: G6PD, G6PD1
• Note: For research use only
• Application notes: Further information: Immunohistochemistry: LS-B3587 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3587 was determined to be 5 ug/ml. Western Blot: Cell lysates of MCF7 (30 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human G6PD (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
• Expiration Date: 12 months from date of receipt.