G-CSF Mouse Monoclonal Antibody

SKU: orb499770

Description

G-CSF Mouse Monoclonal Antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: WB
• Dilution Range: WB=1:500-2000
• Reactivity: Human
• Predicted Reactivity: Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: B4E9
• Immunogen: Recombinded human G-CSF protein
• Target: CSF3
• Molecular Weight: 20 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

C17orf33; CSF3OS; GCSF; CSF3_HUMAN; CSF3; G-CSF; Pluripoietin;

Paraformaldehyde-fixed, paraffin embedded (Mouse liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (G-CSF) Polyclonal Antibody, Unconjugated (orb499770) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Sample: Lane 1: Recombinant human G-CSF protein, His, Primary: Anti-G-CSF (orb499770) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 19 kDa, Observed band size: 20 kDa.

Quick Database Links

Gene Symbol

CSF3

UniProt

P09919