FZD7 Antibody

• Catalog Number: orb1247477
• Category: Antibodies
• Description: FZD7 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Mouse
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-RFYHRLSHSSKGET
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 60kDa band observed in lysates of cell lines HEK293 and HepG2 (calculated MW of 63.6kDa according to NP_003498.1). Recommended concentration: 0.3-1ug/ml.Immunohistochemistry:Paraffin embedded Human Spleen. Recommended concentration: 5ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: FZD7
• UniProt ID: O75084
• NCBI: NP_003498.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: rizzled 7, frizzled homolog 7 (Drosophila), FZD7,
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 60kDa band observed in lysates of cell lines HEK293 and HepG2 (calculated MW of 63.6kDa according to NP_003498.1). Recommended concentration: 0.3-1ug/ml.Immunohistochemistry:Paraffin embedded Human Spleen. Recommended concentration: 5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247477 (0.3 ug/ml) staining of HepG2 cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1247477 (5 ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.