FZD6 Antibody

• Catalog Number: orb1672347
• Category: Antibodies
• Description: FZD6 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, WB
• Predicted Reactivity: Canine, Monkey, Primate
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Anti-FZD6 antibody (orb1672347) was raised against a peptide corresponding to 14 amino acids in the central domain of human FZD6. The immunogen is located within 50-550 amino acids of FZD6.
• Concentration: 1 mg/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 79kD Observed: 95 kD (Post-translational modification: 2 N-linked glycosylations)
• Target: FZD6
• UniProt ID: O60353
• NCBI: O60353
• Storage: FZD6 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: FZD6 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: FZD6 Antibody: Frizzled-6, Fz-6, hFz6
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

WB Validation in Human, Mouse and Rat Cell Lines. Loading: 15 µg of lysate. Antibodies: FZD6 orb1672347, 2 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

Immunofluorescence Validation of FZD6 in Daudi Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed Daudi cells labeling FZD6 with orb1672347 at 10 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Immunofluorescence Validation of FZD6 in Mouse Lung. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse lung labeling FZD6 with orb1672347 at 10 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Immunofluorescence Validation of FZD6 in Rat Lung. Immunofluorescent analysis of 4% paraformaldehyde-fixed rat lung labeling FZD6 with orb1672347 at 10 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).