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Catalog Number | orb2292935 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody raised against a full length recombinant FYN. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 3G11-F9 |
Tested applications | ELISA, PLA, WB |
Reactivity | Human |
Isotype | IgG1 Kappa |
Immunogen | FYN (AAH32496, 1 a.a. ~ 482 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
Conjugation | Unconjugated |
Protein Sequence | MGCVQCKDKEATKLTEERDGSLNQSSGYRYGTDPTPQHYPSFGVTSIPNYNNFHAAGGQGLTVFGGVNSSSHTGTLRTRGGTGVTLFVALYDYEARTEDDLSFHKGEKFQILNSSEGDWWEARSLTTGETGYIPSNYVAPVDSIQAEEWYFGKLGRKDAERQLLSFGNPRGTFLIRESETTKGAYSLSIRDWDDMKGDHVKHYKIRKLDNGGYYITTRAQFETLQQLVQHYSGTWNGNTKVAIKTLKPGTMSPESFLEEAQIMKKLKHDKLVQLYAVVSEEPIYIVTEYMNKGSLLDFLKDGEGRALKLPNLVDMAAQVAAGMAYIERMNYIHRDLRSANILVGNGLICKIADFGLARLIEDNEYTARQGAKFPIKWTAPEAALYGRFTIKSDVWSFGILLTELVTKGRVPYPGMNNREVLEQVERGYRMPCPQDCPISLHELMIHCWKKDPEERPTFEYLQSFLEDYFTATEPQYQPGENL |
NCBI | AAH32496 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | In 1x PBS, pH 7.4 |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Proximity Ligation Analysis of protein-protein interactions between PRKCD and FYN. HeLa cells were stained with anti-PRKCD rabbit purified polyclonal 1:1200 and anti-FYN mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Western Blot analysis of FYN expression in transfected 293T cell line by FYN monoclonal antibody (M01), clone 3G11-F9. Lane 1: FYN transfected lysate (55.43 KDa). Lane 2: Non-transfected lysate.
Western Blot detection against Immunogen (78.76 KDa).