Fyn antibody

• Catalog Number: orb43790
• Category: Antibodies
• Description: Mouse Monoclonal to Fyn.
• Clonality: Monoclonal
• Clone Number: FYN-01
• Tested applications: ICC, IHC-P, IP, WB
• Reactivity: Human, Mouse
• Isotype: Mouse IgG2b
• Immunogen: Bacterially expressed recombinant fragment of human Fyn (aa 7-176).
• Concentration: 1 mg/ml
• Dilution range: Immunohistochemistry (paraffin sections): Recommended dilution: 5 μg/ml; positive tissue: brain. Western blotting: Recommended dilution: 2 μg/ml; positive control: JURKAT human T cell leukemia cell line. Immunoprecipitation: positive control: J77 T cell line. Preparation of cell lysate: 30 min on ice (orbital incubator) in lysing buffer, lysing buffer with N-dodecyl beta-D-maltoside (20 mM Tris/Cl, 100 mM NaCl pH 8,2, 1% laurylmaltosid (w/v), 50 mM NaF). The antibody FYN-01 apparently gives very specific signal (by 59 kDa), and nicely immunoprecipitates Fyn from cell lysates.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: FYN
• Entrez: 2534
• UniProt ID: P06241
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-Fyn antibody
• Note: For research use only
• Application notes: Immunohistochemistry (paraffin sections): Recommended dilution: 5 μg/ml; positive tissue: brain. Western blotting: Recommended dilution: 2 μg/ml; positive control: JURKAT human T cell leukemia cell line. Immunoprecipitation: positive control: J77 T cell line. Preparation of cell lysate: 30 min on ice (orbital incubator) in lysing buffer, lysing buffer with N-dodecyl beta-D-maltoside (20 mM Tris/Cl, 100 mM NaCl pH 8,2, 1% laurylmaltosid (w/v), 50 mM NaF). The antibody FYN-01 apparently gives very specific signal (by 59 kDa), and nicely immunoprecipitates Fyn from cell lysates.
• Expiration Date: 12 months from date of receipt.

Immunoprecipitation of Fyn from the lysate of T cells isolated from fresh buffy coats. Western blot was immunostained with anti-Fyn (FYN-01). Lane 1: original lysate of T cells; Lane 2-3: Immunoprecipitated material eluted from affinity sorbent (FYN-01 coupled to Sepharose beads). Lanes differ in amount of T cell lysate loaded on the immunosorbent.

Immunocytochemistry analysis of Fyn in human primary fibroblasts using anti-Fyn (FYN-01; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue). Primary antibody: 5 ug/ml.

Immunocytochemistry analysis of Fyn in human HeLa cell line using anti-Fyn (FYN-01; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).

Immunocytochemistry analysis of Fyn in murine transformed fibroblasts using anti-Fyn (FYN-01; red). Actin cytoskeleton was decorated by phalloidin (green) and cell nuclei stained with DAPI (blue).