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Catalog Number | orb2292960 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody raised against a partial recombinant FXN. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 3G9 |
Tested applications | ELISA, WB |
Reactivity | Human |
Isotype | IgG1 Kappa |
Immunogen | FXN (AAH48097.1, 91 a.a. ~ 200 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
Conjugation | Unconjugated |
Protein Sequence | DETTYERLAEETLDSLAEFFEDLADKPYTFEDYDVSFGSGVLTVKLGGDLGTYVINKQTPNKQIWLSSPSSGPKRYDWTGKNWVYSHDGVSLHELLAAELTKALKTKLDL |
NCBI | AAH48097.1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | In 1x PBS, pH 7.4 |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western Blot analysis of FXN expression in transfected 293T cell line by FXN monoclonal antibody (M03), clone 3G9. Lane 1: FXN transfected lysate (23.1 KDa). Lane 2: Non-transfected lysate.
Western blot analysis of FXN over-expressed 293 cell line, cotransfected with FXN Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with FXN monoclonal antibody (M03), clone 3G9 (Cat # orb2292960). GAPDH (36.1 kDa) used as specificity and loading control.
Western Blot detection against Immunogen (37.84 KDa).