You have no items in your shopping cart.
Cart summary
Item 1 of 2
Item 1 of 2
FXN / Frataxin Antibody, KO Validated
Catalog Number: orb1274483
| Catalog Number | orb1274483 |
|---|---|
| Category | Antibodies |
| Description | FXN / Frataxin Antibody, KO Validated |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | WB |
| Reactivity | Human, Mouse |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 42-210 of human FXN / Frataxin (NP_000135.2). |
| Antibody Type | Primary Antibody |
| Concentration | batch dependent |
| Form/Appearance | Liquid |
| Conjugation | Unconjugated |
| MW | Observed: 14kDa |
| Target | FXN |
| UniProt ID | Q16595 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | Liquid |
| Alternative names | Frataxin, mitochondrial, Friedreich ataxia protein Read more... |
| Note | For research use only |
| Application notes | WB: 1:500 - 1:2000 |
| Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using FXN/Frataxin antibody (orb1274483) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Western blot analysis of extracts from normal (control) and FXN/Frataxin knockout (KO) 293T cells, using FXN/Frataxin antibody (orb1274483) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
