Ferritin Light Chain Antibody

SKU: orb386090

Description

The FTL gene encodes the light subunit of the ferritin protein. Ferritin is the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. This gene has multiple pseudogenes.Although ferritin light chain has no ferroxidase activity, the light chain may be responsible for the electron transfer across the ferritin protein cage. [Wiki]

Research Area

Epigenetics, Neuroscience

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution range: Western blot: 0.5-2ug/ml,Immunohistochemistry (FFPE): 0.1-0.2ug/ml for 30 min at RT
• Reactivity: Human
• Application Notes:
  Optimal dilution of the Ferritin Light Chain antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2a, kappa
• Clone No.: FTL/1388
• Immunogen: Amino acids 38-165 of human FTL were used as the immunogen for this Ferritin Light Chain antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

IHC testing of FFPE human pancreas with Ferritin Light Chain antibody (clone FTL/1388). Required HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min.

IHC testing of FFPE human pancreas with Ferritin Light Chain antibody (clone FTL/1388). Required HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min.

SDS-PAGE Analysis of Purified, BSA-Free Ferritin Light Chain Antibody (clone FTL/1388). Confirmation of Integrity and Purity of the Antibody.

Western blot testing of human HeLa lysate with Ferritin Light Chain antibody. Predicted molecular weight: ~20 kDa.

Western blot testing of human kidney lysate with Ferritin Light Chain antibody. Predicted molecular weight: ~20 kDa.

Analysis of HuProt (TM) microarray containing more than 19, 000 full-length human proteins using Ferritin Light Chain antibody. These results demonstrate the foremost specificity of the FTL/1388 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Quick Database Links

UniProt

P02792