FTL Antibody / Ferritin light chain

• Catalog Number: orb386089
• Category: Antibodies
• Description: Mammalian ferritins consist of 24 subunits made up of 2 types of polypeptide chains, ferritin heavy chain and ferritin light chain. Ferritin heavy chains catalyze the first step in iron storage, the oxidation of Fe (II), whereas ferritin light chains promote the nucleation of ferrihydrite, enabling storage of Fe (III). Light chain ferritin is involved in cataracts by at least two mechanisms, hereditary hyperferritinemia cataract syndrome, in which light chain ferritin is overexpressed, and oxidative stress, an important factor in the development of ageing-related cataracts.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: FTL/1386
• Tested applications: ELISA, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG2b, kappa
• Immunogen: Amino acids 38-165 of human FTL were used as the immunogen for this Ferritin Light Chain antibody.
• Dilution range: ELISA: order BSA/sodium azide-free format for coating,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.25-0.5ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Ferritin Light Chain antibody is available for research use only.
• UniProt ID: P02792
• Storage: Store the Ferritin Light Chain antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Ferritin Light Chain antibody to be titered up or down for optimal performance.
• Expiration Date: 12 months from date of receipt.

Immunohistochemical staining of Human pancreas using Ferritin (light chain) antibody

Immunohistochemical staining of Human pancreas using Ferritin (light chain) antibody

Western blot analysis of human A431(lane 1), HeLa(lane 2), liver(lane 3) and testis(lane 4) lysate using Ferritin (light chain) antibody