FRMD4B antibody

• Catalog Number: orb2227
• Category: Antibodies
• Description: FRMD4B antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Equine, Mouse, Rabbit, Sheep
• Reactivity: Human, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human FRMD4B (501-600/1034 aa)
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 118 kDa
• Target: FRMD4B
• UniProt ID: Q9Y2L6
• Storage: Shipped at 4°C. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol.
• Alternative names: FERM domain-containing protein 4B; FRM4B_HUMAN; Fr
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: U87MG(Human) Cell Lysate at 30 ug U251(Human) Cell Lysate at 30 ug K562(Human) Cell Lysate at 30 ug Primary: Anti- FRMD4B at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 118 kD Observed

Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min;