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Catalog Number | orb1671521 |
---|---|
Category | Antibodies |
Description | FOXP4 Antibody |
Clonality | Recombinant |
Clone Number | RAB-S40 |
Tested applications | ELISA, FC, IF |
Reactivity | Human |
Isotype | IgG kappa |
Immunogen | This antibody was obtained by recombinant antibody (rAb) phage display recognizing FOXP4 protein under non-denaturing conditions. |
Concentration | batch dependent |
Conjugation | Unconjugated |
Target | FOXP4 |
UniProt ID | Q8IVH2 |
Storage | Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. |
Buffer/Preservatives | PBS with 0.02% Proclin 300. |
Alternative names | Fork head-related protein-like A, Forkhead box pro Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Flow cytometry using the anti-FOXP4 antibody. HepG2 cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (3.0; isotype control - black line) or the r1 version (blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
Immunofluorescence staining of fixed HeLa cells with anti-FOXP4 antibody. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix™ coverslips stained with the chimeric r version at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue) and the actin stain is phalloidin (red). Panels show from left-right- top-bottom orb1671521- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.
Western Blot using anti-FOXP4 antibody Nuclear lysate of K562 cells (35 ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rsion at 0.01 ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.
IHC, WB | |
Animal, Bovine, Canine, Guinea pig, Human, Mouse, Porcine, Rabbit, Rat | |
Canine, Equine, Guinea pig, Human, Mouse, Porcine, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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