FoxO1 (phospho-Ser256) antibody

• Catalog Number: orb6050
• Category: Antibodies
• Description: FoxO1 (phospho-Ser256) antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC-P
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Porcine
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human FOXO1 around the phosphorylation site of Ser256 (AA(p-S)MD)
• Concentration: 1mg/ml
• Dilution range: IHC-P=1:100-500, Flow-Cyt=1μg /Test, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 72 kDa
• Target: Phospho-FoxO1 (Ser256)
• UniProt ID: Q12778
• Storage: Shipped at 4°C. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol.
• Alternative names: FOXO1A (phospho S256); p-FOXO1A (phospho S256); FK
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraffin-embedded rat pancreas tissue fixed with 4% paraformaldehyde. Antigen retrieval by boiling with citrate buffer. Blocking buffer is goat serum (37 degrees for 20 min.). FoxO1 (phospho-Ser256) antibody at 1:200 dilution with overnight incubation.