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Catalog Number | orb18857 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to FOXI1 |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Human |
Dilution range | ELISA: 1:2000, WB: 1-3 μg/ml |
Conjugation | Unconjugated |
MW | 41.0; 30.8 |
Target | FOXI1 / FKHL10 |
Entrez | 2299 |
Protein Sequence | TSGVLYPREGTEV |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti FOXI1 antibody, anti forkhead box I1 antibody Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: Immunohistochemistry: This product has been successfully used in Mouse Epididymus (Blomqvist et al, EMBO J. 2006 Sep 6;25(17):4131-41. PMID 16932748).WB: Preliminary experiments gave bands at approx 50 kDa and 25 kDa in Human Kidney lysate after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the predicted sizes of 41.0kDa according to NP_036320 (isoform a) and 30.8kDa according to NP_65892 (isofrom b). The bands were successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported |
Expiration Date | 12 months from date of receipt. |
orb18857 Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.
orb18857 (6µg/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
orb18857 (1µg/ml) staining of Human Testes lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Immunohistochemical staining of Human Testis using FOXI1 antibody
WB analysis of Human Testes lysate using FOXI1 antibody
ELISA, FC, ICC, IF, IHC, WB | |
Human, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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