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Catalog Number | orb18539 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to FOXE1 |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IHC |
Reactivity | Human |
Dilution range | ELISA: 1:8000, WB: 0.3 μg/ml, IHC-P: 5 μg/ml |
Conjugation | Unconjugated |
MW | 38.1 |
Target | FOXE1 / TTF2 |
Entrez | 2304 |
Protein Sequence | AYPGGIDRFVSAM |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti FOXE1 antibody, anti TTF2 antibody, anti fork Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Testis shows strong nuclear staining in developing sperm cells. Recommended concentration, 2-3ug/ml.This product has also been successfully used in Human Thyroid Gland: Zhang et al, Pathol Int. 2006 May;56(5):240-5.PMID: 166WB: Preliminary experiments gave an approx 70kDa band in Human Thymus and Thyroid Gland lysates after 0.3 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 38.1kDa according to NP_004464.2. The 65kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported |
Expiration Date | 12 months from date of receipt. |
Immunohistochemical staining of Human Thyroid Gland using FOXE1 antibody
Immunohistochemical staining of Human Testis using FOXE1 antibody
ELISA, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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