FOXA2 antibody

• Catalog Number: orb18541
• Category: Antibodies
• Description: Goat polyclonal antibody to FOXA2
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human, Mouse, Rat
• Dilution range: ELISA: 1:4000, WB: 1-3 μg/ml, IHC-P: 4-8 μg/ml
• Conjugation: Unconjugated
• MW: 48.9; 48.3
• Target: FOXA2 / HNF3B
• Entrez: 3170
• Protein Sequence: GVYSRPIMNSS
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti FOXA2 antibody, anti HNF3B antibody, anti for
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.WB: Approx 60kDa band observed in Human Liver lysates (calculated MW of 48.3kDa according to NP_068556.1and NP_710141.1). The observed molecular weight corresponds to earlier findings in literature (Wang et al, J Biol Chem. 2002 May 17;277(20):17564-70.; PMID: 11875061). Recommended concentration: 1-3 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb18541 staining (1ug/ml) of Human Liver lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

orb18541 (1µg/ml) staining of Rat Lung lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence

orb18541 (3.8µg/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.