FOXA1 Antibody [FOXA1/1241]

• Catalog Number: orb1252170
• Category: Antibodies
• Description: FOXA1 Antibody [FOXA1/1241]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: FOXA1/1241
• Tested applications: FC, IF, IHC-P, WB
• Reactivity: Human, Rat
• Isotype: IgG2b, kappa
• Immunogen: A human recombinant partial protein corresponding to amin acids 372-472 was used as the immunogen for this FOXA1 antibody.
• Antibody Type: Primary Antibody
• Concentration: 0.2 mg/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: FOXA1
• UniProt ID: P55317
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: FOXA1 Antibody: HNF3A, TCF3A, HNF3A, Hepatocyte nu
• Note: For research use only
• Application notes: Flow Cytometry: 0.5-1ug/10^6 cellsIF: 0.5-1 ug/mlWB: 0.5-1 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 min at RTThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the FOXA1 antibody to be titered up or down for optimal performance.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human prostate carcinoma with FOXA1 antibody (clone FOXA1/1241). HIER: boil sections in 10mM Tris with 1mM EDTA, pH9 for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE human bladder carcinoma with FOXA1 antibody (clone FOXA1/1241). HIER: boil sections in 10mM Tris with 1mM EDTA, pH9 for 10-20 min followed by cooling at RT for 20 min.

SDS-PAGE Analysis of Purified, BSA-Free FOXA1 Antibody (clone FOXA1/1241). Confirmation of Integrity and Purity of the Antibody.