FOXA1 Antibody

SKU: orb1252170

Description

FOXA1 Antibody

Images & Validation

• Tested Applications: FC, IF, IHC-P, WB
• Reactivity: Human, Rat
• Application Notes:
  Flow Cytometry: 0.5-1ug/10^6 cellsIF: 0.5-1 µg/mLWB: 0.5-1 µg/mLIHC (FFPE): 0.5-1 µg/mL for 30 min at RTThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the FOXA1 antibody to be titered up or down for optimal performance.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b, kappa
• Clone No.: FOXA1/1241
• Immunogen: A human recombinant partial protein corresponding to amin acids 372-472 was used as the immunogen for this FOXA1 antibody.
• Target: FOXA1
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

FOXA1 Antibody: HNF3A, TCF3A, HNF3A, Hepatocyte nuclear factor 3-alpha, Forkhead box protein A1, HNF-3-alpha

IHC testing of FFPE human prostate carcinoma with FOXA1 antibody (clone FOXA1/1241). HIER: boil sections in 10mM Tris with 1mM EDTA, pH9 for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE human bladder carcinoma with FOXA1 antibody (clone FOXA1/1241). HIER: boil sections in 10mM Tris with 1mM EDTA, pH9 for 10-20 min followed by cooling at RT for 20 min.

SDS-PAGE Analysis of Purified, BSA-Free FOXA1 Antibody (clone FOXA1/1241). Confirmation of Integrity and Purity of the Antibody.

Quick Database Links

Gene Symbol

FOXA1

UniProt

P55317