Fos B/FOSB Antibody

• Catalog Number: orb312091
• Category: Antibodies
• Description: Fos B/FOSB Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC, WB
• Predicted Reactivity: Hamster
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Fos B (264-291aa LPFQTSQDAPPNLTASLFTHSEVQVLGD), different from the related mouse sequence by two amino acids.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 35928 MW
• UniProt ID: P53539
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Protein fosB;G0/G1 switch regulatory protein 3;FOS
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of SiHa cells using anti-FOSB antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of FOSB using anti-FOSB antibody.Lane 1:rat thymus tissue; 2:mouse thymus tissue; 3:mouse ovary tissue; 4:mouse spleen tissue; 5:mouse kidney tissue; 6:mouse RAW246.7 cell; 7:human HL-60 cell; 8:human THP-1 cell.

IHC analysis of FOSB using anti-FOSB antibody. FOSB was detected in paraffin-embedded section of human renal cancer tissue.

IHC analysis of FOSB using anti-FOSB antibody. FOSB was detected in paraffin-embedded section of human placenta tissue.

IHC analysis of FOSB using anti-FOSB antibody. FOSB was detected in paraffin-embedded section of mouse intestine tissue.

IHC analysis of FOSB using anti-FOSB antibody. FOSB was detected in paraffin-embedded section of rat intestine tissue.