Fos B Rabbit Monoclonal Antibody

• Catalog Number: orb547788
• Category: Antibodies
• Description: Fos B Rabbit Monoclonal Antibody
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: DFO-6
• Tested applications: ICC, IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthesized peptide derived from human Fos B
• Concentration: Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
• Dilution range: WB 1:500-1:2000IHC 1:50-1:200ICC/IF 1:50-1:200IP 1:20
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 35928 MW
• UniProt ID: P53539
• Storage: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
• Alternative names: Protein fosB;G0/G1 switch regulatory protein 3;FOS
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunohistochemical analysis of paraffin-embedded mouse brain, using Fos B Antibody (orb547788) FOSB was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-FOSB Antibody (orb547788) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # orb90444) with DAB as the chromogen.

Western blot analysis of FosB expression in C6 cell lysate treated with serum (orb547788). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOSB monoclonal antibody (Catalog # orb547788) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90503) with Tanon 5200 system.

IF analysis of Fos B using anti-Fos B antibody (orb547788) and anti-Beta Tubulin antibody (orb623835). Fos B was detected in immunocytochemical section of HELA cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (orb90553) for 15 mins. The cells were blocked with 10% goat serum. And then incubated at 1: 50 with rabbit anti-Fos B Antibody (orb547788) and mouse anti-Beta Tubulin antibody (orb623835) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (orb27705) and DyLight®488 Conjugated Goat Anti-Mouse IgG were used as secondary antibody at 1: 500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.