FOLR4/Juno Protein, Human, Recombinant (His)

• Catalog Number: orb1960314
• Category: Proteins
• Description: Izumo1 is the only essential sperm-egg fusion protein currently known on mammalian sperm, and its egg receptor (Juno; formerly Folr4) was recently discovered. Male knockout mice for Izumo1 and female knockout mice for Juno are both healthy but sterile. Here, both sperm-egg binding proteins are shown to be evolving under positive selection. Juno's presence in mammals alone, suggesting a recent mammalian-specific duplication and neofunctionalization of the ancestral folate receptor. FOLR4/Juno Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 25 kDa and the accession number is A6ND01-1.
• Tag: C-His
• Purity: 98.00%
• MW: 25 kDa (predicted). Due to glycosylation, the protein migrates to 30-35 kDa based on Tris-Bis PAGE result.
• UniProt ID: A6ND01
• Expression System: HEK293 Cells
• Biological Origin: Human
• Biological Activity: Izumo1 is the only essential sperm-egg fusion protein currently known on mammalian sperm, and its egg receptor (Juno; formerly Folr4) was recently discovered. Male knockout mice for Izumo1 and female knockout mice for Juno are both healthy but sterile. Here, both sperm-egg binding proteins are shown to be evolving under positive selection. Juno's presence in mammals alone, suggesting a recent mammalian-specific duplication and neofunctionalization of the ancestral folate receptor. FOLR4/Juno Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 25 kDa and the accession number is A6ND01-1.
• Expression Region: Gly20-Ser228
• Storage: -20°C
• Note: For research use only
• Application notes: Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
• Expiration Date: 6 months from date of receipt.