Folate Hydrolase 1 Antibody / FOLH1 / PSMA

• Catalog Number: orb2642694
• Category: Antibodies
• Description: Folate hydrolase 1 (FOLH1), also known as Prostate-specific membrane antigen (PSMA), is a type II transmembrane glycoprotein belonging to the M28 peptidase family. FOLH1 has two enzymatic activities, one as a prostate-specific integral membrane folate hydrolase and the other as a carboxypeptidase In the prostate the protein is up-regulated in cancerous cells and is used as an effective diagnostic and prognostic indicator of prostate cancer.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: FOLH1/2363
• Tested applications: IHC-P, WB
• Reactivity: Bovine, Canine, Human, Rat
• Isotype: Mouse IgG2b, kappa
• Immunogen: A portion of amino acids 232-433 from the human protein was used as the immunogen for this FOLH1 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Western blot: 0.5-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This FOLH1 antibody is available for research use only.
• UniProt ID: Q04609
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the FOLH1 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human prostate cancer with FOLH1 antibody. Required HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

IHC testing of FFPE human prostate carcinoma with FOLH1 antibody. Required HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

Western blot testing of human LNCaP cell lysate with FOLH1 antibody. Predicted molecular weight ~100 kDa.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using FOLH1 antibody. These results demonstrate the foremost specificity of the FOLH1/2363 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free FOLH1 antibody as confirmation of integrity and purity.

IHC testing of FFPE rat kidney tissue with FOLH1 antibody. Required HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

IHC testing of FFPE dog kidney tissue with FOLH1 antibody. Required HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

IHC testing of FFPE cow brain tissue with FOLH1 antibody. Required HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.