Focal Adhesion Kinase (phospho-Y925) antibody

• Catalog Number: orb214479
• Category: Antibodies
• Description: Rabbit polyclonal antibody to PTK2
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat, Zebrafish
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y925 of human Focal Adhesion Kinase protein. The exact sequence is proprietary.
• Dilution range: WB: 1-500-1-1000, IF/ICC: 1-100-1-500
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: PTK2
• Entrez: 14083, 5747, 25614
• UniProt ID: Q05397, P34152, O35346
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti FAK antibody, anti FAK1 antibody, anti Focal
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Focal Adhesion Kinase (Phospho-Y925) expression in NIH3T3 pervanadate-treated (A) whole cell lysates. (Predicted band size: 119; 99; 63; 48; 120; 39; 114 kD; Observed band size: 125 kD)

Immunofluorescent analysis of Focal Adhesion Kinase (Phospho-Y925) staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.