Focal Adhesion Kinase (phospho-Y861) antibody

• Catalog Number: orb214478
• Category: Antibodies
• Description: Rabbit polyclonal antibody to PTK2
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y861 of human Focal Adhesion Kinase protein. The exact sequence is proprietary.
• Dilution range: WB: 1-500-1-1000, IP: 1-10-1-100
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: PTK2
• Entrez: 5747
• UniProt ID: Q05397
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti FAK antibody, anti FAK1 antibody, anti Focal
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Focal Adhesion Kinase (Phospho-Y861) expression in mouse kidney (A), rat kidney (B) whole cell lysates. (Predicted band size: 119; 99; 63; 48; 120; 39; 114 kD; Observed band size: 100 kD)

Immunohistochemical analysis of Focal Adhesion Kinase (Phospho-Y861) staining in human kidney cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.