FN1 antibody

• Catalog Number: orb765230
• Category: Antibodies
• Description: Rabbit polyclonal antibody to FN1
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human Fibronectin 1. AA range:2337-2386
• Concentration: 1 mg/ml
• Conjugation: Unconjugated
• MW: 260
• Storage: Store at -20°C. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-FN1 antibody, anti-FN antibody, anti-Fibronec
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of A549. 1, primary Antibody (red) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min.

Western blot analysis of lysates from 1) 3T3, 2) KB , 3) K562 , 4) 293T cells, (Green) primary antibody was diluted at 1:1000, 4°C over night, secondary antibody was diluted at 1:10000, 37°C 1 hour. (Red) Actin β Monoclonal Antibody (5B7) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody was diluted at 1:10000, 37°C 1 hour.

Western Blot analysis of various cells using FN1 Polyclonal Antibody diluted at 1:2000.

Western Blot analysis of HeLa cells using FN1 Polyclonal Antibody diluted at 1:2000.