FN1 Antibody

• Catalog Number: orb1258909
• Category: Antibodies
• Description: FN1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 2200-2355 of human Fibronectin (NP_002017.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:1000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 290kDa
• Target: FN1
• UniProt ID: P02751
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: FN1, CIG, DKFZp686F10164, DKFZp686H0342, DKFZp686I
• Note: For research use only
• Application notes: WB: 1:500 - 1:1000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using Fibronectin antibody (orb1258909) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded human lung cancer using Fibronectin antibody (orb1258909) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse testis leydig using Fibronectin antibody (orb1258909) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using Fibronectin antibody (orb1258909) at dilution of 1:100. Blue: DAPI for nuclear staining.