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Catalog Number | orb533142 |
---|---|
Category | Tools |
Description | Fluorescein-12-dCTP is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incorporated as substitute for its natural counterpart dCTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of cytidine. Recommended Fluorescein-12-dCTP/dCTP ratio for PCR and Nick Translation: 30-50% Fluorescein-12-dCTP-/70-50% dCTP. |
Concentration | 1.0 mM-1.1 mM |
Form/Appearance | solution in 10 mM Tris-HCl; Color: yellow-orange; pH: 7.5 ± 0.5 |
Purity | ≥ 95% (HPLC) |
MW | Theoretical MW: 991.68 g/mol; Detected MW: 991.15 g/mol |
SMILES | [C@@H]1(C[C@@H](O[C@@H]1COP(=O)(OP(=O)(OP(=O)(O)O)O)O)n1c(=O)nc(c(c1)C#CCNC(=O)CCCCCNC(=O)c1ccc(c(c1)c1c2c(oc3c1ccc(=O)c3)cc(cc2)O)C(=O)O)N)O.[C@@H]1(C[C@@H](O[C@@H]1COP(=O)(OP(=O)(OP(=O)(O)O)O)O)n1c(=O)nc(c(c1)C#CCNC(=O)CCCCCNC(=O)c1ccc(c(c1)C(=O)O)c1c2c(oc3c1ccc(=O)c3)cc(cc2)O)N)O |
Formula | C39H40N5O20P3 |
Storage | store at -20 °C |
Note | For research use only |
Application notes | Incorporation into DNA/cDNA by: PCR with Taqpolymerasein-house data; Nick Translation with DNAse I/DNA Polymerase Iin-house data.
Spectroscopic Propertie: λexc 492 nm, λem 517 nm, ε 83.0 L mmol-1 cm-1 (Tris-HCl pH 7.5). |
Expiration Date | 12 months from date of receipt. |
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