FLNA antibody

• Catalog Number: orb216108
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Filamin A (Phospho-S2152)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Rat
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S2152 of human Filamin A protein. The exact sequence is proprietary.
• Dilution range: WB: 1:500-1:1000, IHC-P: 1:100-1-200, IP: 1:10-1:100
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: FLNA
• Entrez: 2316
• UniProt ID: P21333
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti FLN antibody, anti FLN1 antibody, anti Filami
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Filamin A (Phospho-S2152) expression in HeLa calyculin A-treated (A), A431 EGF-treated (B) whole cell lysates. (Predicted band size: 280 kD; Observed band size: 280 kD)

Immunohistochemical analysis of Filamin A (Phospho-S2152) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.