FLLL32

SKU: orb1300420

Description

FLLL32 is a potent small molecule inhibitor of the JAK2/STAT3 signaling pathway, demonstrating an IC50 value below 5 μM. It is widely used in biochemical and cellular research to study oncogenesis, inflammation, and immune responses, with applications in both in vitro assays and in vivo animal models of cancer.

Research Area

Cardiovascular Research, Cell Biology, Epigenetics & Chromatin, Signal Transduction, Stem Cell & Developmental Biology

Key Properties

• CAS Number: 1226895-15-3
• MW: 464.55
• Purity: 97.00%
• Formula: C₂₈H₃₂O₆
• SMILES: COc1ccc(\C=C\C(=O)C2(CCCCC2)C(=O)\C=C\c2ccc(OC)c(OC)c2)cc1OC
• Target: STAT,Apoptosis,JAK
• Solubility: DMSO:85 mg/mL (182.97 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:3.3 mg/mL (7.1 mM);Ethanol:24 mg/mL (51.66 mM);H2O:< 1 mg/mL (insoluble or slightly soluble)

Bioactivity

• Target IC50:
  JAK2:<5 μM
• In Vivo:
  In MDA-MB-231 xenografted mice, FLLL32 (50 mg/kg, i.p.) significantly reduces tumor burdens. In mouse xenografts with OS-33 osteosarcoma cells, FLLL32 (50 mg/kg, i.p.) also inhibits tumor growth by targeting STAT3.
• In Vitro:
  In MDA-MB-231 breast and PANC-1 pancreatic cancer cells, FLLL32 downregulates STAT3 phosphorylation and DNA-binding activity. In MDA-MB-231 cells, FLLL32 inhibit the formation of colonies and cell invasion. In human multiple myeloma, glioblastoma, liver cancer, and colorectal cancer cell lines, FLLL32 also leads to the inhibition of cell proliferation and the induction of caspase-3 and PARP cleavages.
• Cell Research:
  Cells are seeded in 96-well plates (3,000 per well) in triplicate and then treated with 0.5 to 5 μM of FLLL31 or FLLL32 or with 0.5 to 30 μM of curcumin for 72 h. MTT (25 μL) is added to each sample and incubated for 3.5 h. Then, 100 μL of N,N-dimethylformamide solubilization solution are added to each well. The absorbance at 450 nm is read the following day. IC50 are determined using Sigma Plot 9.0 software.(Only for Reference)

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Inhibitor, FLLL 32, FLLL32, FLLL-32, inhibit, JAK, JAK2, Janus kinase, Apoptosis, STAT

Compound Identifiers

PubChem CID

68019246