FLG antibody

• Catalog Number: orb393053
• Category: Antibodies
• Description: Rabbit polyclonal antibody to FLG.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Filaggrin. The exact sequence is proprietary.
• Dilution range: WB: 1:500:1000
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: FLG
• Entrez: 2312
• UniProt ID: P11088, P20930
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: Filaggrin
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Filaggrin expression in Myla2059 (A) whole cell lysates. (Predicted band size: 435 kD; Observed band size: 435 kD)

Immunohistochemical analysis of Filaggrin staining in human skin formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. AEC was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.