You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb1257306 |
---|---|
Category | Antibodies |
Description | FIS1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-122 of human TTC11 / FIS1 (NP_057152.2). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 15kDa |
Target | FIS1 |
UniProt ID | Q9Y3D6 |
Storage | Store at -20°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
Alternative names | Mitochondrial Fission 1 Protein; Tetratricopeptide Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using TTC11/FIS1 antibody (orb1257306) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Immunohistochemistry of paraffin-embedded rat heart using TTC11/FIS1 antibody (orb1257306) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using TTC11/FIS1 antibody (orb1257306) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using TTC11/FIS1 antibody (orb1257306) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using TTC11/FIS1 antibody (orb1257306) at dilution of 1:50 (60x lens). Blue: DAPI for nuclear staining.
ICC, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, ICC, IF, IHC, WB | |
Human, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
Filter by Rating