FIPI

SKU: orb1304037

Description

FIPI is a potent dual inhibitor of phospholipase D isoforms PLD1 and PLD2, with IC50 values of 25 nM and 20 nM, respectively. It is widely used in research to study PLD's role in cytoskeletal dynamics, cell migration, and chemotaxis in both cellular and animal models of cancer and inflammation.

Research Area

Cell Biology, Metabolism Research

Key Properties

• CAS Number: 939055-18-2
• MW: 421.47
• Purity: 99.08%
• Formula: C₂₃H₂₄FN₅O₂
• SMILES: Fc1ccc2[nH]c(cc2c1)C(=O)NCCN1CCC(CC1)n1c2ccccc2[nH]c1=O
• Target: Autophagy,Phospholipase
• Solubility: DMSO:12.5 mg/mL (29.66 mM)

Bioactivity

• Target IC50:
  PLD1:25 nM|PLD2:20 nM
• In Vitro:
  FIPI is a potent, concentration-dependent PLD2 inhibitor, and we show here that it inhibits PLD1 equally well under standard in vitro assay conditions. FIPI was added into the cell culture media 1 h before performing an in vivo PLD assay and was found to be a potent inhibitor of PLD2 with an IC50 of 10 nM. The typical localization of PLD1 to peri-nuclear membrane vesicles and PLD2 to the plasma membrane were not affected by exposure to FIPI and and FIPI did not decrease PIP2 availability on the plasma membrane in PLD1- and PLD2-overexpressing cells as assessed using an enhanced GFP-fused PIP2 sensor. FIPI did not significantly inhibit p38 or ERK phosphorylation in bone marrow-derived macrophages stimulated with lipopolysaccharide. FIPI inhibition of PLD did diminish fMLP-directed chemotaxis(p < 0.01), validating this role for PLD function and suggesting that PLD regulates chemotaxis via mechanisms distinct from affecting MAKP signaling.
• Cell Research:
  Cytotoxicity in MAECs is determined by assaying the extent of reduction in MTT in intact cells using the commercial MTT reduction assay kit. At the end of the experimental treatments, MTT solution (10% vol/vol in MEM) is added and the cells are incubated for 3 hours, following which MTT solvent is added in an amount equal to the original culture volume. Absorbance of the reduced MTT is determined spectrophotometrically, according to the manufacturer's recommendations.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Inhibitor, inhibit, PLD2, PLD1, FIPI, Autophagy, 5-Fluoro-2-indolyl deschlorohalopemide, Phospholipase

Compound Identifiers

PubChem CID

16739265