Fibronectin, mouse Antibody

SKU: orb27336

Description

Rabbit polyclonal antibody to Fibronectin

Research Area

Musculoskeletal & Connective Tissue Research

Images & Validation

• Tested Applications: ELISA, IF, IHC, IHC-P, RIA
• Dilution Range: IHC (frozen): 1:1000, ELISA:1:20000 (OD 0.5) Optimal working dilutions should, however, be determined by antibody titration.
• Reactivity: Mouse
• Application Notes:
  Positive Control: Mouse skin. Pre-Treatment: After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 min at 37°C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended. Incubation Time: IHC 60 min at RT or 2-8°C over night

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Purified fibronectin from murine plasma
• Purification: Protein G affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Concentration: app. 5 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Images on the left: Specific indirect immunostaining of connective tissue in frozen sections of mouse skin with orb27336 (diluted 1:1000) Images on the right: Corresponding DAPI staining of nuclei in epidermis, muscle and connective tissue.

Quick Database Links

UniProt

P11276