Fibronectin, mouse

• Catalog Number: orb27336
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Fibronectin
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC, IHC-P, RIA
• Reactivity: Mouse
• Immunogen: Purified fibronectin from murine plasma
• Antibody Type: Primary Antibody
• Concentration: app. 5 mg/ml
• Dilution range: IHC (frozen): 1:1000, ELISA:1:20000 (OD 0.5) Optimal working dilutions should, however, be determined by antibody titration.
• Conjugation: Unconjugated
• Hazard Information: These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. It should be handled only by trained staff.
• UniProt ID: P11276
• Source: Serum from rabbits immunized with purified mouse fibronectin.
• RRID: AB_10749739
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Protein G affinity purification
• Note: For research use only
• Application notes: Positive Control: Mouse skin. Pre-Treatment: After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg e.g. Art. No. HYA02-50) in TBS 15 min at 37°C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended. Incubation Time: IHC 60 min at RT or 2-8°C over night
• Expiration Date: 12 months from date of receipt.

Images on the left: Specific indirect immunostaining of connective tissue in frozen sections of mouse skin with orb27336 (diluted 1:1000) Images on the right: Corresponding DAPI staining of nuclei in epidermis, muscle and connective tissue.