Fibronectin Antibody

• Catalog Number: orb607339
• Category: Antibodies
• Description: Mouse monoclonal antibody to Fibronectin
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: Fn-3
• Tested applications: FC, IF, IHC
• Reactivity: Human
• Isotype: IgG1
• Immunogen: FR5 cells, derived by SV40 transformation of human mammary epithelial cells.
• Concentration: Purified Ab with BSA and Azide at 200ug/ml
• Dilution range: Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes);Optimal dilution for a specific application should be determined.
• Form/Appearance: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Conjugation: Unconjugated
• MW: 220kDa (monomer); 440kDa (dimer)
• Target: Fn1
• Entrez: 2335
• UniProt ID: P02751
• Expression System: Bioreactor
• Storage: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
• Buffer/Preservatives: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Alternative names: Cold insoluble globulin (CIG); FINC; FN1; FNZ; GFN
• Note: For research use only
• Application notes: Positive Control: SW156 cells. Kidney
• Expiration Date: 12 months from date of receipt.