Fibroblast growth factor 2 FGF2 Antibody

• Catalog Number: orb48039
• Category: Antibodies
• Description: Fibroblast growth factor 2 FGF2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, ICC, IF, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human FGF2 recombinant protein (Position: P143-S288).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat ELISA , 0.1-0.5μg/ml, Human, -Western blot, 0.1-0.5μg/ml, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 30770 MW
• UniProt ID: P09038
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Fibroblast growth factor 2;FGF-2;Basic fibroblast
• Note: For research use only
• Application notes: By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of SiHa cells using anti-FGF2 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.

WB analysis of FGF2 using anti-FGF2 antibody.Lane 1:human HeLa cell;2:human SKOV3 cell;3:human SiHa cell; 4:human u87 cell.

IF analysis of FGF2 using anti-FGF2 antibody. FGF2 was detected in an immunocytochemical section of SiHa cells.