FHIT Antibody (monoclonal, 26H7)

• Catalog Number: orb421119
• Category: Antibodies
• Description: FHIT Antibody (monoclonal, 26H7)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 26H7
• Tested applications: FC, ICC, IF, WB
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: E.coli-derived human FHIT recombinant protein (Position: M1-Q147). Human FHIT shares 90% and 87% amino acid (aa) sequence identity with mouse and rat FHIT, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml Immunocytochemistry/Immunofluorescence, 2μg/ml Flow Cytometry, 1-3μg/1x106 cells
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 17 kDa
• UniProt ID: P49789
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Bis (5'-adenosyl)-triphosphatase; AP3A hydrolase;
• Note: For research use only
• Application notes: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of 293T cells using anti-FHIT antibody (Blue line).Isotype control antibody (Green line) was mouse IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of FHIT using anti-FHIT antibody.Lane 1:human COLO-320 cell;2:human SW620 cell;3:human SGC-7901 cell.

IF analysis of FHIT using anti-FHIT antibody. FHIT was detected in immunocytochemical section of MCF7 cells.