FGF4 Antibody

• Catalog Number: orb1272774
• Category: Antibodies
• Description: FGF4 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hFGF-4. Human FGF-4 specific antibody was purified by affinity chromatography employing immobilized hFGF-4 matrix.
• Concentration: batch dependent
• Dilution range: ELISA:Indirect:To detect hFGF-4 by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hFGF-4.SandwichTo detect hFGF-4 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human FGF-4 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hFGF-4. Western Blot:To detect hFGF-4 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFGF-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• Target: FGF4
• UniProt ID: P08620
• NCBI: P08620
• Storage: FGF-4 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
• Alternative names: HST, KFGF, HST-1, HSTF1, K-FGF, HBGF-4, HST, KS3,
• Note: For research use only
• Application notes: ELISA:Indirect:To detect hFGF-4 by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hFGF-4.SandwichTo detect hFGF-4 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human FGF-4 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hFGF-4. Western Blot:To detect hFGF-4 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFGF-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
• Expiration Date: 12 months from date of receipt.

This antibody stained formalin-fixed paraffin-embedded sections of normal breast tissue from a breast carcinoma. The recommended concentration is 0.25 ug/ml with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer is recommended. Optimal concentrations and conditions may vary.