FGA monoclonal antibody (M01), clone 1D12

• Catalog Number: orb2293068
• Category: Antibodies
• Description: Mouse monoclonal antibody raised against a full-length recombinant FGA.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG2a Kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: In 1x PBS, pH 7.4
• Immunogen: FGA (AAH20764, 1 a.a. ~ 218 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Protein Sequence: MFSMSIVCLVLSVVGTAWTADSGEGDFLAEGGGVRGPRVVERHQSACKDSDWPFCSGEDWNYKCPSGCRMKGLIDEVNQDFTNRINKLKNSLFEYQKNNKDSHSLTTNIMEILRGDFSSANNRDNTYNRVSEDLRSRIEVLKRKVIEKVTANNLLVARVTTEETPHLKARAIKWQMRPEVKPIMKEHIAPREAMLNLALSEVSTLLLWGSLPCPPRLS
• Tested applications: ELISA, IP, PLA, WB
• Clone Number: 1D12
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• NCBI: AAH20764

Detection limit for recombinant GST tagged FGA is 1 ng/ml as a capture antibody.

Immunoprecipitation of FGA transfected lysate using anti-FGA monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with FGA MaxPab rabbit polyclonal antibody.

Proximity Ligation Analysis of protein-protein interactions between APOA1 and FGA. HeLa cells were stained with anti-APOA1 rabbit purified polyclonal 1:1200 and anti-FGA mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Western Blot analysis of FGA expression in transfected 293T cell line by FGA monoclonal antibody (M01), clone 1D12. Lane 1: FGA transfected lysate (24.7 KDa). Lane 2: Non-transfected lysate.

Western Blot detection against Immunogen (49.72 KDa).