FFAR3 antibody

• Catalog Number: orb214002
• Category: Antibodies
• Description: Rabbit polyclonal antibody to FFAR3 also known as G-protein coupled receptor41 whose expression can be detected in the sympathetic ganglia and is highly expressive in the adipose tissues. It is found in the cell membrane. This protein controls the production of hormones involved in whole-body energy homeostasis, for instance it regulates blood pressure through renin secretion and may also regulate secretion of the PYY peptide by enteroendocrine cells and controls gut motility, intestinal transit rate, and the harvesting of energy from SCFAs produced by gut microbiota. It can also mediate the activation of the inflammatory and immune response by SCFAs in the gut regulating the rapid production of chemokines and cytokines by intestinal epithelial cells.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human GPR41. The exact sequence is proprietary.
• Dilution range: WB: 1-500-1-1000
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: FFAR3
• Entrez: 233080, 2865
• UniProt ID: Q3UFD7, O14843
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti-GPR41 antibody, anti-Free fatty acid receptor
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of GPR41 expression in rat liver (A) whole cell lysates. (Predicted band size: 38 kD; Observed band size: 39 kD)

Immunofluorescent analysis of GPR41 staining in LOVO cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.