Ferret IgA (alpha chain) antibody (HRP)

• Catalog Number: orb1463544
• Category: Antibodies
• Description: Ferret IgA (alpha chain) antibody (HRP)
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Ferret
• Isotype: IgG
• Immunogen: Anti-Ferret IgA was produced by repeated immunization with ferret IgA alpha heavy chain in goat.
• Concentration: 1.0 mg/ml
• Dilution range: ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:5,000
• Form/Appearance: Lyophilized
• Purity: Anti-Ferret IgA (alpha chain) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Ferret IgA coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Ferret IgA and Ferret Serum. Specificity was confirmed by ELISA at less than 1% cross reactivity against other Ferret heavy or light chain isotypes.
• Conjugation: HRP
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Thimerosal
• Alternative names: goat anti-Ferret IgA (alpha chain) Antibody peroxi
• Note: For research use only
• Application notes: Anti-Ferret IgA Peroxidase conjugate is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency. Ferret IgA (alpha chain) antibody has been assayed against 1.0 ug of Ferret IgA in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for Anti-Ferret IgA (alpha chain) Antibody.
• Expiration Date: 12 months from date of receipt.