FEN1 antibody

• Catalog Number: orb1474290
• Category: Antibodies
• Description: Mouse monoclonal antibody to FEN1
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: IF, WB
• Reactivity: Hamster, Human, Mouse, Primate, Rat
• Immunogen: Purified recombinant human FEN-1 protein fragments expressed in E.coli.
• Dilution range: WB (1/500 - 1/1000), IF/IC (1/50 - 1/100)
• Form/Appearance: Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3.
• Conjugation: Unconjugated
• Target: FEN1
• Entrez: 84490, 2237
• UniProt ID: Q5XIP6, P39748, P39749
• Source: Mouse
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3.
• Alternative names: RAD2; Flap endonuclease 1; FEN-1; DNase IV; Flap s
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of FEN1 expression in Hela (A), Jurkat (B), NIH3T3 (C), COS7 (D), PC12 (E), C6 (F), Raw264.7 (G) whole cell lysates. (Predicted band size: 43 kD; Observed band size: 43 kD)

Immunofluorescent analysis of FEN1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.