FCCP

SKU: orb1300423

Description

FCCP is a potent mitochondrial uncoupler that dissipates the proton gradient, inhibiting oxidative phosphorylation. It is widely used in research to induce apoptosis and study mitochondrial function, metabolism, and cellular bioenergetics in both in vitro and in vivo models.

Research Area

Cell Biology, Metabolism Research, Pharmacology & Drug Discovery, Signal Transduction

Key Properties

• CAS Number: 370-86-5
• MW: 254.17
• Purity: 99.65%
• Formula: C₁₀H₅F₃N₄O
• SMILES: FC(F)(F)Oc1ccc(NN=C(C#N)C#N)cc1
• Target: Mitochondrial Metabolism,PTEN,ATPase,OXPHOS
• Solubility: DMSO:55 mg/mL (216.39 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2.55 mg/mL (10.03 mM)

Bioactivity

• Target IC50:
  T47D cells:0.31 μM|mitoCHO cellsndrial oxidative pHOS cellsphate:20 nM
• In Vivo:
  METHODS: To test the effect on stroke, FCCP (1 mg/kg) was injected intraperitoneally into a C57BL/6J mouse model of stroke, followed by one hour of transient middle cerebral artery occlusion (tMCAO). RESULTS: Infarct volumes in the cortex, striatum, and whole hemisphere were significantly increased in mice pretreated with FCCP. Mice receiving FCCP had significantly increased neurologic deficit scores compared to carriers. METHODS: To assay anti-tumor activity in vivo, FCCP (1 mg/kg) and cisplatin (2 mg/kg) were intraperitoneally injected every two days for two weeks into C57BL/6 mice harboring mouse ovarian epithelial carcinoma tumor ID8. RESULTS: The combination of FCCP and cisplatin inhibited tumor growth via OMA1-induced mitochondrial and ER stress.
• In Vitro:
  METHODS: Rat ventricular myocytes were treated with FCCP (10-1000 nM) for 1400 sec, and cellular oxygen consumption was measured using a Clark Oxygen Electrode. RESULTS: Oxygen consumption increased significantly, immediately, and in a dose-dependent manner after the addition of FCCP. METHODS: Rat adrenal medullary chromaffinoma cells PC12 were treated with FCCP (30 µM) for 0.5-2 h. The rate of protein synthesis was measured using [3H]methionine. RESULTS: FCCP treatment produced a strong inhibition (68%) of protein synthesis rate for at least 2 hours.
• Cell Research:
  Protein synthesis rate is assayed in 24-mm diameter multi-well dishes with fresh medium containing 0.175 Ci/mmol of [3H]methionine (200 μM), for 30 min at 37°C. PC12 cells are treated with FCCP for different period of times. (Only for Reference)

Storage & Handling

• Storage: keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone, ATPase, FCCP, Inhibitor, MitochondrialMetabolism, Mitochondrial Metabolism, inhibit, Trifluoromethoxy carbonylcyanide phenylhydrazone

Compound Identifiers

PubChem CID

3330

A Seifert, H Vekaria, E Aryee Characterization of mitochondrial membrane potential protocols.io, (2025)

PubMed: