FBXO5 Antibody

• Catalog Number: orb1252065
• Category: Antibodies
• Description: FBXO5 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: FBXP5
• Tested applications: IF, IHC-P, WB
• Reactivity: Human
• Isotype: IgG2a, kappa
• Immunogen: A human partial recombinant N-terminal protein fragment was used as the immunogen for the EMI-1 antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Western blot: 1-2 ug/mlImmunofluorescence: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)Optimal dilution of the EMI-1 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 minutes2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: FBXO5
• UniProt ID: Q9UKT4
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: F-box only protein 5, Early mitotic inhibitor 1, F
• Note: For research use only
• Application notes: Western blot: 1-2 ug/mlImmunofluorescence: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)Optimal dilution of the EMI-1 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 minutes2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Western blot testing of human 1) HeLa and 2) 293 lysate with EMI-1 antibody. Predicted molecular weight: 50/45 kDa (isoforms 1/2).

IHC testing of FFPE human ovarian carcinoma and EMI-1 antibody (FBXP5).