Fatty Acid Synthase/FASN Antibody

• Catalog Number: orb334598
• Category: Antibodies
• Description: Fatty Acid Synthase/FASN Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived human FASN recombinant protein (Position: M1-E226). Human FASN shares 90.3% and 90.7% amino acid (aa) sequence identity with mouse and rat FASN, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, By Heat Immunocytochemistry,0.5-1μg/ml Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 273427 MW
• UniProt ID: P49327
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Fatty acid synthase;2.3.1.85;[Acyl-carrier-protein
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of FASN using anti-FASN antibody.Lane 1:human HeLa cell;2:human HepG2 cell;3:human hepatocellular carcinoma tumor tissue (HCCT) lysates;4:human 293T cell;5:human MCF-7 cell;6:human RT4 cell;7:human A549 cell.

WB analysis of FASN using anti-FASN antibody.Lane 1:rat liver tissue;2:mouse lung tissue;3:mouse liver tissue;4:mouse NIH/3T3 cell.

IF analysis of FASN using anti-FASN antibody. FASN was detected in a paraffin-embedded section of human ovarian cancer tissue.

IF analysis of FASN using anti-FASN antibody. FASN was detected in a paraffin-embedded section of human rectal cancer tissue.

IF analysis of FASN using anti-FASN antibody. FASN was detected in an immunocytochemical section of T47D cells.

IHC analysis of FASN using anti-FASN antibody. FASN was detected in a paraffin-embedded section of human intestinal cancer tissue.