FAT4 Antibody

• Catalog Number: orb1520252
• Category: Antibodies
• Description: FAT4 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human
• Immunogen: between 4931 and 4981
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 8-15 µl/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: FAT4
• UniProt ID: Q6V0I7
• NCBI: Q6V0I7.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: FATJ; putative protein product of Nbla00548; proto
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human FAT4 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and KM12 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-FAT4 antibody (orb1520252) used for WB at 0.4 mg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

Detection of human FAT4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-FAT4 antibody (orb1520252) used for IP at 6 µg per reaction.