FAT2 Antibody

• Catalog Number: orb1252213
• Category: Antibodies
• Description: FAT2 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 8C5
• Tested applications: FC, IF, IHC, WB
• Reactivity: Human
• Isotype: IgG1, kappa
• Immunogen: A recombinant human Protocadherin Fat 2 fusion protein was used as the immunogen for this FAT2 antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RTTitering of the FAT2 antibody may be required for optimal performance.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: FAT2
• UniProt ID: Q9NYQ8
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Note: For research use only
• Application notes: Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 ug/mlIHC (FFPE): 0.5-1 ug/ml for 30 minutes at RTTitering of the FAT2 antibody may be required for optimal performance.
• Expiration Date: 12 months from date of receipt.

IHC testing of human cervical carcinoma with FAT2 antibody (clone 8C5). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

IHC testing of human bladder carcinoma with FAT2 antibody (clone 8C5). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

SDS-PAGE Analysis of Purified, BSA-Free FAT2 Antibody (clone 8C5). Confirmation of Integrity and Purity of the Antibody.